Evaluation of ferritin and TfR level in plasma neural-derived exosomes as potential markers of Parkinson’s disease

Introduction Early diagnosis of Parkinson’s disease (PD) remains challenging. It has been suggested that abnormal brain iron metabolism leads to excessive accumulation in PD, although the mechanism deposition is not yet fully understood. Ferritin and transferrin receptor (TfR) are involved metabolism, exosome pathway one by which ferritin transported regulated. While blood healthy animals contains a plentiful supply TfR-positive exosomes, no studies have examined TfR plasma neural-derived exosomes. Methods Plasma exosomes were obtained from 43 patients with PD 34 controls. Neural-derived isolated anti-human L1CAM antibody immunoabsorption. Transmission electron microscopy western blotting used identify ELISAs quantify levels Receivers operating characteristic (ROC) curves applied map diagnostic accuracy TfR. Independent predictors identified using logistic regression models. Results exhibited typical exosomal morphology expressed specific marker CD63. significantly higher than controls (406.46 ± 241.86 vs. 245.62 165.47 ng/μg, P = 0.001 1728.94 766.71 1153.92 539.30 < 0.001, respectively). There significant positive correlations between control group, group all individuals (rs 0.744, 0.700, 0.752, The level was independently associated (adjusted odds ratio 1.002; 95% CI 1.000–1.003). ROC performances ferritin, TfR, their combination moderate (0.730, 0.812, 0.808, However, relationship found biomarkers progression. Conclusion hypothesized may be potential for they participate PD.